Jingyue Ju

SAMUEL RUBEN-PETER G. VIELE PROFESSOR OF ENGINEERING AND PROFESSOR OF CHEMICAL ENGINEERING AND PHARMACOLOGY DIRECTOR, CENTER FOR GENOME TECHNOLOGY & BIOMOLECULAR ENGINEERING

806 Northwest Corner Building
Mail Code 48-28

Tel(212) 851-9191

Jingyue Ju’s research uses molecular science and engineering to create and develop new approaches for highly efficient and cost-effective DNA sequencing, an essential technology in bringing humanity closer to the goal of personalized precision medicine.

Research Interests

Genomics Science and Engineering, Chemical and Biological Engineering.

Research Areas

The Ju Laboratory focuses on the design and synthesis of novel molecules for biological engineering, imaging, and the development of new technologies for genomic research. His team uses a combination of chemical science, engineering principles, and experimental biological approaches to pursue these research objectives. Ju pioneered the application of chemistry and fluorescence energy transfer (ET) principle to construct ET molecular tags for high-throughput DNA sequencing and analysis; this fluorescent labeling technology was widely used for the completion of the international Human Genome Project.

At Columbia University, the Ju Laboratory invented several generations of DNA sequencing technologies, including the design and synthesis of cleavable fluorescent nucleotide reversible terminators that enabled the development of the DNA sequencing by synthesis platform, currently the dominant approach used in next-generation sequencing systems, which laid the foundation for the $1,000 genome. Ju is the inventor of 39 U.S. patents in genomic technology, including a novel method for single molecule real-time electronic DNA sequencing, which has the potential to accelerate the use of DNA sequencing for wide applications in clinical diagnosis and precision medicine.

The Ju Laboratory has established extensive collaborations with colleagues at Columbia University and biotechnology and pharmaceutical companies to implement advancements in genome science and technology for large-scale sequencing projects, the investigation of epigentics and disease gene discovery initiatives. A project of particular distinction involves the use of genomic approaches to decipher memory circuits for the study of brain function, which will help delineate the molecular mechanisms underlying neurological disorders.

Ju received a B.S. in Chemistry from Inner Mongolia University in 1985, an M.S. in Chemistry from the Dalian Institute of Chemical Physics, Chinese Academy of Sciences in 1988, and a Ph.D. in Chemistry from the University of Southern California in 1994. He was a U.S. Department of Energy Human Genome Distinguished Postdoctoral Fellow at the University of California at Berkeley from 1994 to 1995. He was a recipient of Packard Fellowship in Science and Engineering.

RESEARCH EXPERIENCE

  • Postdoctoral fellow, University of California at Berkeley, 1994-1995

PROFESSIONAL EXPERIENCE

  • Samuel Ruben-Peter G. Viele Professor of Engineering, Columbia University, 2011–Present
  • Professor of Chemical Engineering and Pharmacology, Columbia University, 2010–Present
  • Director, Center for Genome Technology and Biomolecular Engineering, Columbia University, 2003–Present
  • Professor of Chemical Engineering and Head of DNA Sequencing and Chemical Biology, Columbia Genome Center, Columbia University, 2005–Present
  • Associate Professor of Chemical Engineering and Head of DNA Sequencing and Chemical Biology, Columbia Genome Center, Columbia University, 1999–2005
  • Senior Scientist & Director, Chemistry & Assay Development, Incyte Genomics, Inc., 1995–1999

HONORS & AWARDS

  • Distinguished Fundamental Research Award for Next Generation DNA Sequencing Research & Development. Next Generation Sequencing (NGS) Research and Development Association, China, 2017
  • Packard Fellowship in Science and Engineering, 2001
  • U.S. Department of Energy Human Genome Distinguished Postdoctoral Fellow, 1994 

SELECTED PUBLICATIONS

  • Real-Time Single-Molecule Electronic DNA Sequencing by Synthesis Using Polymer-Tagged Nucleotides on a Nanopore Array”. C. W. Fuller, S. Kumar, M. Porel, M. Chien, A. Bibillo, P. B. Stranges, M. Dorwart, C. Tao, Z. Li, W. Guo, S. Shi, D. Korenblum, A. Trans, A. Aguirre, E. Liu, E. Harada, J. Pollard, A. Bhat, C. Cech, A. Yang, C. Arnold, M. Palla, J. S. Hovis, R. Chen, I. Morozova, S. Kalachikov, J. J. Russo, J. Kasianowicz, R. Davis, S. Roever, G. M. Church, and J. Ju. Proceedings of the National Academy of Sciences USA. 2016, 113, 5233-5238.
  • DNA Sequencing by Synthesis Using 3′-O-azidomethyl Nucleotide Reversible Terminators and Surface-Enhanced Raman Spectroscopic Detection”. M. Palla, W. Guo, S. Shi, Z. Li, J. Wu, S. Jockusch, C. Guo, J.J. Russo, N.J. Turro and J. Ju. Royal Society of Chemistry Advances, 2014, 4, 49342-49346.
  • A Strategy to Capture and Characterize the Synaptic Transcriptome”. S.V. Puthanveettil, I. Antonov, S. Kalachikov, P. Rajasethupathy, Y.B. Choi, A.B. Kohn, M. Citarella, F. Yu, K.A. Karl, M. Kinet, I. Morozova, J.J. Russo, J. Ju, L.L. Moroz, E.R. Kandel. Proceedings of the National Academy of Sciences USA. 2013, 110, 7464-7469.
  • PEG-labeled Nucleotides and Nanopore Detection for Single Molecule DNA Sequencing by Synthesis”. S. Kumar, C. Tao, M. Chien, B. Hellner, A. Balijepalli, J. W. F. Robertson, Z. Li, J. J. Russo, J. E. Reiner, J. J. Kasianowicz, and J. Ju. Scientific Reports. 2012, 2, 684, 1-8.
  • Four-Color DNA Sequencing by Synthesis Using Cleavable Fluorescent Nucleotide Reversible Terminators”. J. Ju, D. Kim, L. Bi, Q. Meng, X. Bai, Z. Li, X. Li, M.S. Marma, S. Shi, J. Wu, J.R. Edwards, A. Romu, N.J. Turro. Proceedings of the National Academy of Sciences USA. 2006, 103, 19635-19640.
  • A Novel Class of Small RNAs Bind to MILI Protein in Mouse Testes”. Aravin, D. Gaidatzis, S. Pfeffer, M. Quintana, P. Morris, S. Kuramochi-Miyagawa, T. Nakano, M. Chien, J. J. Russo, J. Ju, R. Sheridan, C. Sander, M. Zavolan & T. Tuschl. Nature. 2006, 442, 203-207.
  • Neuronal Transcriptome of Aplysia: Neuronal Compartments and Circuitry”. L.L. Moroz, J.R. Edwards, S.V. Puthanveettil, A. Kohn , T. Ha, A. Heyland, B. Kudsen, A. Sahni , F. Yu,  L. Liu, S. Jezzini, R. Sadreyev, P. Lovell, W. Iannucculli, M. Chen, T. Nguyen, H. Sheng, R. Shaw, S. Kalachikov, Y. Panchin, W. Farmerie, J.J. Russo, J. Ju, E.R. Kandel. Cell. 2006, 127, 1453-1467.
  • The Genomic Sequence of the Accidental Pathogen Legionella Pneumophila”. M. Chien, I. Morozova, S. Shi, H. Sheng, J. Chen, S.M. Gomez, G. Asamani, K. Hill, J. Nuara, M. Feder, J. Rineer, J.J. Greenberg, V. Steshenko, S.H. Park, B. Zhao, E. Teplitskaya, J.R. Edwards, S. Pampou, A. Georghiou, I.C. Chou, W. Iannuccilli, M.E. Ulz, D.H. Kim, A. Geringer-Sameth, C. Goldsberry, P. Morozov, S.G. Fischer, G. Segal, X. Qu, A. Rzhetsky, P. Zhang, E. Cayanis, P.J. De Jong, J. Ju, S. Kalachikov, H.A. Shuman, J.J. Russo. Science. 2004, 305, 1966-1968.
  • Digital Genotyping Using Molecular Affinity and Mass Spectrometry” S. Kim, H. D. Ruparel, T. C. Gilliam, and J. Ju. Nature Reviews Genetics. 2003, 4, 1001-1008.
  • Combinatorial Fluorescence Energy Transfer Tags for Multiplex Biological Assays” A. K. Tong, Z. Li, G. S. Jones, J. J. Russo, J. Ju. Nature Biotechnology. 2001, 19, 756-759.